@Article{SatoRMMRMVLBB:2021:RoNaMo,
               author = "Sato, Tabata P. and Rodrigues, Bruno V. M. and Mello, Daphne C. R. 
                         and M{\"u}nchow, Eliseu A. and Ribeiro, Juliana S. and Machado, 
                         Jo{\~a}o Paulo Barros and Vasconcellos, Luana M. R. and Lobo, 
                         Anderson de Oliveira and Bottino, Marco C. and Borges, Alexandre 
                         L. S.",
          affiliation = "{Universidade Estadual Paulista (UNESP)} and {Universidade Brasil} 
                         and {Universidade Estadual Paulista (UNESP)} and {Universidade 
                         Federal do Rio Grande do Sul (UFRGS)} and {University of Michigan 
                         School of Dentistry} and {Instituto Nacional de Pesquisas 
                         Espaciais (INPE)} and {Universidade Estadual Paulista (UNESP)} and 
                         {Universidade Brasil} and {University of Michigan School of 
                         Dentistry} and {Universidade Estadual Paulista (UNESP)}",
                title = "The role of nanohydroxyapatite on the morphological, physical, and 
                         biological properties of chitosan nanofibers",
              journal = "Clinical Oral Investigations",
                 year = "2021",
               volume = "25",
               number = "5",
                pages = "3095--3103",
                month = "May",
             keywords = "Antimicrobial, Chitosan, Electrospinning, Regeneration, 
                         Regenerative dentistry, Scaffolds.",
             abstract = "Objectives: This study aimed to evaluate the effects of 
                         nanohydroxyapatite (nHAp) particles on the morphological, 
                         chemical, physical, and biological properties of chitosan 
                         electrospun nanofibers. Materials and methods: nHAp particles with 
                         a 1.67 Ca/P ratio were synthesized via the aqueous precipitation 
                         method, incorporated into chitosan polymer solution (0.5 wt%), and 
                         electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan 
                         fibers (nHAp-free, Ch fibers) were used as the control. The 
                         electrospun fiber mats were characterized using morphological, 
                         topographical, chemical, thermal, and a range of biological 
                         (antibacterial, antibiofilm, cell viability, and alkaline 
                         phosphatase [ALP] activity) analyses. Data were analyzed using 
                         ANOVA and Tukeys test (\α = 0.05). Results: ChHa fibers 
                         demonstrated a bead-like morphology, with thinner (331 ± 110 nm) 
                         and smoother (Ra = 2.9 ± 0.3 \μm) distribution as compared 
                         to the control fibers. Despite showing similar cell viability and 
                         ALP activity to Ch fibers, the ChHa fibers demonstrated greater 
                         antibacterial potential against most tested bacteria (except for 
                         P. intermedia), and higher antibiofilm activity against P. 
                         gingivalis biofilm. Conclusions: The incorporation of nHAp 
                         particles did not jeopardize the overall morphology, topography, 
                         physical, and biological characteristics of the chitosan 
                         nanofibers. Clinical relevance: The combination of nHAp particles 
                         with chitosan can be used to engineer bioactive, electrospun 
                         composite nanofibers with potential applications in regenerative 
                         dentistry. © 2020, Springer-Verlag GmbH Germany, part of Springer 
                         Nature.",
                  doi = "10.1007/s00784-020-03633-6",
                  url = "http://dx.doi.org/10.1007/s00784-020-03633-6",
                 issn = "1432-6981",
             language = "en",
           targetfile = "Sato_role.pdf",
        urlaccessdate = "20 maio 2024"
}